Journal: Advanced healthcare materials

Article Title: CLDN18.2 CAR-derived Extracellular Vesicle Immunotherapy Improves Outcome in Murine Pancreatic Cancer.

doi: 10.1002/adhm.202500546

Figure Lengend Snippet: Figure 1. Illustration of CAR-T cell-derived CAR-sEV production and cytotoxicity of parental CAR-T cells. A) Schematic representation of CAR-sEV secre- tion from CLDN18.2 CAR-T cells. B) Diagram illustrating the design of the CLDN18.2 CAR constructs. C) Flow cytometric analysis showing CLDN18.2 expression on KPC-derived murine primary pancreatic cancer cells, CHX1990 and CHX2000, in adherent (left) and spheroid (right) cultures. D) Cytotoxic effects of murine CLDN18.2 CAR-T cells on GFP+ primary KPC pancreatic cancer cells, including CLDN18.2+ CHX2000 WT, CLDN18.2−CHX2000 KO, and CLDN18.2−CHX1990, in the presence of untransduced T cells (UTD) and CLDN18.2 CAR-T cells at varying effector-to-target (E:T) ratios, with representative images shown. E) IFN-𝛾production by CLDN18.2 CAR-T cells in response to their exposure to target cells for 24 h as assessed by FC. F) LDH release as a readout for cell death is shown for 12, 24, and 48 h of co-culture. G) Release of IFN-𝛾(upper panel) and granzyme B (lower panel) by CAR-T cells in response to their exposure to target cells as assessed by ELISA.

Article Snippet: Plasma IL-6 levels were measured using the High Sensitivity Mouse IL-6 ELISA Kit (Elabscience, E-HSEL-M0003) according to the manufac- turer’s instructions.

Techniques: Derivative Assay, Construct, Expressing, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

Journal: Advanced healthcare materials

Article Title: CLDN18.2 CAR-derived Extracellular Vesicle Immunotherapy Improves Outcome in Murine Pancreatic Cancer.

doi: 10.1002/adhm.202500546

Figure Lengend Snippet: Figure 6. Efficacy and safety profiles of CLDN18.2 CAR-sEVs and CAR-T cells. A) Schematic of the in vivo treatment protocol using CLDN18.2 CAR-T cells or CLDN18.2 CAR-sEVs in an orthotopic PDAC model. B) Bioluminescence imaging of tumor-bearing mice at the indicated time points. C) Quantification of tumor burden based on total photon flux. D) Kaplan–Meier survival analysis comparing CAR-sEVs and CAR-T cells; statistical significance determined by log-rank test. E) IL-6 levels in plasma collected on day 1 and 10 post-treatment as measured by ELISA.

Article Snippet: Plasma IL-6 levels were measured using the High Sensitivity Mouse IL-6 ELISA Kit (Elabscience, E-HSEL-M0003) according to the manufac- turer’s instructions.

Techniques: In Vivo, Imaging, Clinical Proteomics, Enzyme-linked Immunosorbent Assay